P8232

Anti-P2X₇ Purinergic Receptor antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• Synonym(e): Anti-P2X₇ Antibody, P2X₇ Receptor Detection, Rabbit Anti-P2X₇
• MDL-Nummer: MFCD02097118
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: lyophilized powder
• Speziesreaktivität: human, mouse, rat
• Methode(n): immunohistochemistry: suitable; western blot: 1:200-1:1,000
• UniProt-Hinterlegungsnummer: Q99572
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... P2RX7(5027) ; mouse ... P2rx7(18439) ; rat ... P2rx7(29665)

Beschreibung

Allgemeine Beschreibung

Purinergic receptor P2X₇ is an ATP-gated cation channel. It is expressed in retinal pigmented epithelium and neural retina. The gene encoding this protein is localized on human chromosome 12.

Immunogen

Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 Receptor (Accession Q64663). Intracellular, C-terminus.

Anwendung

Anti-P2X7 Purinergic Receptor antibody produced in rabbit has been used in

• immunoprecipitation
• immunofluorescence and confocal microscopy
• western blotting

Biochem./physiol. Wirkung

Purinergic receptor P2X₇ has a role in immunological processes. It has a role during oxidative stress under pathological conditions. The channel is permeable to Na⁺, Ca²⁺ and K⁺. Prolonged activation of the channel under some pathological conditions leads to the passage of large molecules and ultimately cell death. P2X7 may be involved in the release of interleukin-1β (IL-1β) and indirectly initiate an inflammatory cascade.

Physikalische Form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide.

Lagerung und Haltbarkeit

Prior to reconstitution, store at –20°C. After reconstitution, the stock antibody solution may be stored at 2–8°C for up to two weeks. For extended storage, freeze at -20°C, or below, in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. Centrifuge all antibody preparations before use (10,000 x g 5 min). Working dilution samples should be discarded if not used within 12 hours.

Sonstige Hinweise

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable