P4932

Polymyxin B -sulfat (Salz)

powder, non-animal origin, suitable for cell culture, BioReagent

• Synonym(e): Polymixin, Polymixin B, Polymixin B sulfate
• Empirische Formel (Hill-System): C₅₅H₉₆N₁₆O₁₃ · 2H₂SO₄
• CAS-Nummer: 1405-20-5
• Molekulargewicht: 1385.61
• EG-Nummer: 215-774-7
• MDL-Nummer: MFCD00131991
• UNSPSC-Code: 12352207
• NACRES: NA.76

Eigenschaften

• product name: Polymyxin B -sulfat (Salz), powder, BioReagent, suitable for cell culture
• Produktlinie: BioReagent
• Qualitätsniveau: 200
• Form: powder
• Wirksamkeit: ≥6,000 USP units per mg
• Methode(n): cell culture | mammalian: suitable
• Wirkungsspektrum von Antibiotika: Gram-negative bacteria
• Wirkungsweise: cell membrane | interferes
• Lagertemp.: 2-8°C
• SMILES String: O=C(C(NC(C(CCN)NC(CCCCC(C)CC)=O)=O)C(C)O)NC(CCN)C(NC(CCNC(C(NC(C(NC(C(CCN)N1)=O)CCN)=O)C(O)C)=O)C(NC(CCN)C(NC(CC2=CC=CC=C2)C(NC(CC(C)C)C1=O)=O)=O)=O)=O.O=S(O)(O)=O
• InChI: 1S/C48H82N16O13.H2O4S/c1-27(2)24-37-47(76)59-32(11-19-52)41(70)56-31(10-18-51)43(72)61-35(14-22-65)39(68)54-21-13-34(45(74)57-33(12-20-53)44(73)64-38(48(77)63-37)25-28-6-4-3-5-7-28)60-42(71)30(9-17-50)58-46(75)36(15-23-66)62-40(69)29(8-16-49)55-26-67;1-5(2,3)4/h3-7,26-27,29-38,65-66H,8-25,49-53H2,1-2H3,(H,54,68)(H,55,67)(H,56,70)(H,57,74)(H,58,75)(H,59,76)(H,60,71)(H,61,72)(H,62,69)(H,63,77)(H,64,73);(H2,1,2,3,4)
• InChIKey: HNDFYNOVSOOGDU-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Polymyxin B is a member of the polymyxin group of antibiotics, obtained from different strains of Bacillus polymyxa and related species. It contains a cyclic heptapeptide unit and a tripeptide side chain, which is N-acylated by a fatty acyl residue. Polymyxin B sulfate salt is generally used for the treatment of Gram-negative bacterial infections.
Chemical structure: peptide

Anwendung

Polymyxin B sulfate salt has been used:

• for the treatment of BAC-1.2F5 murine macrophages as an LPS (lipopolysaccharide) inhibitor
• as positive and negative control during microbicidal concentration (MCC) assay

Biochem./physiol. Wirkung

Polymyxin B sulfate has been used clinically to treat infections of the urinary tract, meninges and blood stream caused by susceptible strains of Pseudomonas aeruginosa. It also has been used in studying multidrug-resistant pathogens, as an immobilized agent for removal of endotoxins, and to induce pore formation in the membranes of cortex cells from excised sorghum roots.

Wirkungsweise: Polymyxin-B-Sulfat bindet an den Lipid-A-Teil der bakteriellen Lipopolysaccharide† und unterbricht die Zytoplasmamembran durch Bildung von Poren, die groß genug sind, um einen Nukleotidaustritt durch die Bakterienwände zu ermöglichen. Dadurch wird die Durchlässigkeit der Zytoplasmamembran gestört.

Antimikrobielles Spektrum: Hat eine bakterizide Wirkung auf die meisten gramnegativen Stäbchen††, einschließlich E. coli, sowie auf die meisten Pilze und grampositiven Bakterien.

Vorsicht

As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions. Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.

Angaben zur Herstellung

Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

Sonstige Hinweise

1mu,5mu

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302
• P-Sätze: P264 - P270 - P301 + P312 - P501
• Gefahreneinstufungen: Acute Tox. 4 Oral
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves