P3862

Anti-phospho-PKB (pThr³⁰⁸) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-phospho-Akt
• MDL-Nummer: MFCD02686506
• UNSPSC-Code: 12352203
• NACRES: NA.44

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 56 kDa
• Speziesreaktivität: rat, mouse
• Methode(n): microarray: suitable; western blot: 1:1,000 using a whole extract of PDGF-treated mouse fibroblast NIH3T3 cells and a whole extract of H₂O₂-treated rat fibroblast Rat-1 cells
• UniProt-Hinterlegungsnummer: P31750
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: phosphorylation (pThr308)
• Angaben zum Gen: human ... AKT1(207) ; mouse ... Akt1(11651) ; rat ... Akt1(24185)

Beschreibung

Allgemeine Beschreibung

Protein kinase B (PKB, also known as Akt) is a serine/threonine protein kinase and a key regulator of signal transduction processes. Important processes such as cell cycle, proliferation and apoptosis are regulated by PKB in response to stimuli such as growth factors, insulin and cytokines. Three isoforms of PKB have been identified, α, β and γ. PKB α is reportedly overexpressed in breast cancer cell lines, whereas PKB β is overexpressed in ovarian and pancreatic cancers. On cell stimulation by growth factors or insulin, PKB α translocates to the cell membrane, where it undergoes full activation by phosphorylation at Thr³⁰⁸ and Ser 473, by PDK1 and PDK2. PKB α plays a crucial role in several downstream pathways involved in glycogen synthesis, protein translation and cell proliferation. PKB is also a potent inhibitor of apoptotic cell death by suppressing BAD-induced death
Anti-phospho PKB (pThr³⁰⁸) recognises PKB phosphorylated at threonine 308 (56 kDa).

Immunogen

synthetic phosphopeptide corresponding to the pThr³⁰⁸ region of human PKBα (amino acids 301-315).

Anwendung

Anti-phospho PKB (pThr³⁰⁸) may be used for detection and localization of phospho-PKB by immunoblotting. A minimum dilution of 1:1000 may be used for detection by immunoblotting in PDGF-treated mouse NIH3T3 cells and peroxide-treated Rat-1 fibroblast cell line.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable