OGS3157

PSF-OXB20-NH2-PELB - PELB SECRETION PLASMID

plasmid vector for molecular cloning

• Synonym(e): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• UNSPSC-Code: 12352200
• NACRES: NA.85

Eigenschaften

• Rekombinant: expressed in E. coli
• Form: buffered aqueous solution
• Mol-Gew.: size 3914 bp
• Bakterienauswahl: kanamycin
• Replikationsursprung: pUC (500 copies)
• Peptidspaltung: no cleavage
• Lage der Peptid-Tags: N-terminal
• Promoter: Promoter name: OXB20; Promoter activity: constitutive; Promoter type: bacterial
• Reportergen: none
• Sekretionssignal: PelB
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an Pectate Lyase B (PelB) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Sequenz

To view sequence information for this product, please visit the product page

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable