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OGS1367

PSF-CMV-PURO-NH2-KRYFP - N-TERMINAL YFP TAG MAMMALIAN PLASMID

Name: PSF-CMV-PURO-NH2-KRYFP - N-TERMINAL YFP TAG MAMMALIAN PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

Synonym(e):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

UNSPSC-Code:

12352200

NACRES:

NA.85

Markierung

yellow fluorescent protein (YFP) tagged

Form

buffered aqueous solution

Mol-Gew.

size 6854 bp

Bakterienauswahl

kanamycin

Säugerzellenauswahl

puromycin

Replikationsursprung

pUC (500 copies)

Peptidspaltung

no cleavage

Lage der Peptid-Tags

N-terminal

Promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

Reportergen

YFP

Versandbedingung

ambient

Lagertemp.

−20°C

Allgemeine Beschreibung

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains an n-terminal non-aequorea based yellow fluorescent protein (KrYFP) reporter tag that can be fused to a gene of interest to allow protein detection.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Lagerklassenschlüssel

12 - Non Combustible Liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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