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Merck

N5408

Sigma-Aldrich

Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse

clone ME20.4, ascites fluid

Synonym(e):

Anti-NGFR p75

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About This Item

MDL-Nummer:
UNSPSC-Code:
51111800
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

ME20.4, monoclonal

Mol-Gew.

antigen 75 kDa

Enthält

15 mM sodium azide

Speziesreaktivität

racoon, monkey, pig, human, feline

Darf nicht reagieren mit

mouse, chicken, rat

Methode(n)

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tongue
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
neutralization: suitable
radioimmunoassay: suitable
western blot: suitable

Isotyp

IgG1

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... NGFR(4804)

Allgemeine Beschreibung

Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) (mouse IgG1 isotype) is derived from the ME20.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a human melanoma cell line WM245. The human NGFR p75 has a hydrophobic signal sequence, a single N-linked glycosylation site, four cysteine-rich repeat units of approximately 40 amino acids in the extracellular domain, a serine- and threonine-rich region which might be O-linked glycosylated, a single transmembrane domain, and a 155-amino acid cytoplasmic domain.
Recognizes the low affinity human NGFR p75 (also known as gp75NGFR , p75NGFR and p75NTR). The epitope recognized by the antibody exists within the third or fourth cysteine-rich repeats of the NGFR molecule.

Spezifität

Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) recognizes the low affinity NGFR p75 of human origin (75 kDa), also known as gp75NGFR, p75NGFR and p75NTR .The epitope recognized by the antibody is located within the third or fourth cysteine-rich repeats of the NGFR molecule. Cross-reactivity has been observed with human, monkey (marmoset), pig, cat and raccoon , but not with rat, chicken or mouse NGFR.

Immunogen

human melanoma cell line WM245.

Anwendung

Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse has been used in immunoblotting. to added in CB.
Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) may be used:
  • for enzyme linked immune sorbent assay (ELISA)
  • immunoprecipitation, immunoblotting and immunohistochemistry
  • for immunocytochemistry (acetone- or formalin-fixed, Bouin′s solution - or formalin- fixed paraffin-embedded)
  • for electron- microscopy radioimmunoassay (RIA) (Kd of 7x10-10 M)
  • flow cytometry
  • for the inhibition of binding of NGF to the receptor
  • for rossette assay
  • as a cholinergic immunotoxin

Biochem./physiol. Wirkung

Nerve Growth Factor Receptor (NGFR) p75 enhances the NGF binding affinity of the proto-oncogene product p140trk and also may modulate the kinase activity of p140trk and play a role in signal transduction. Specifically, neurotrophin binding to NGFR p75 stimulates generation of ceramide, activation and translocation of nuclear factor- κ (NF-κB) to the nucleus, and enhancement of Jun kinase (JNK) activity.
Neurotrophins (NT) are growth factors that are essential for the development and maintenance of the nervous system. One of the receptors that mediate the effects of NTs is the Nerve Growth Factor Receptor/TNFRSF16 (p75NGFR, p75NTR). NGFR mediates the effects of NGF that are important in the survival and differentiation of neurons and neural crest-derived cells, during embryonic and early postnatal development. Expression of NGFR has been associated with favorable prognosis and survival in breast and pancreatic cancers.

Physikalische Form

The product is supplied as ascites fluid with 15 mM sodium azide as a preservative.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Origin and immunolesioning of cholinergic basal forebrain innervation of cat primary auditory cortex
Kamke MR, et al.
Hearing Research, 206(1-2) (2005)
The intracellular domain of the low affinity p75 nerve growth factor receptor is a death effector domain
Park HH
Molecular Medicine Reports, 2(4), 539-541 (2009)
Expression and structure of the human NGF receptor
Johnson D, et al.
Cell, 47(4), 545-554 (1986)
Chang-Gyu Hahn et al.
The Journal of comparative neurology, 483(2), 154-163 (2005-01-29)
The birth and differentiation of neurons have been extensively studied in the olfactory epithelium (OE) of rodents but not in humans. The goal of this study was to characterize cellular composition and molecular expression of human OE in vivo and
A Fine et al.
Neuroscience, 81(2), 331-343 (1997-09-23)
Four groups of monkeys (Callithrix jacchus) were injected with saline or increasing amounts of the immunotoxin, ME20.4 IgG-saporin, directly into the basal nucleus of Meynert via a frontal trajectory which avoided damage to the overlying basal ganglia. ME20.4 IgG binds

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