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N5162

Sigma-Aldrich

Anti-Neurabin II antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Anti-Neural-tissue Specific F-actin Binding Protein I, Anti-PP1bp134, Anti-Protein Phosphatase 1 Regulatory Subunit 9B, Anti-Spinophilin

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About This Item

MDL-Nummer:
MFCD02686629
UNSPSC-Code:
12352203

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen 140 kDa

Speziesreaktivität

rat, mouse, canine

Methode(n)

immunoprecipitation (IP): 5-10 μg using rat brain extract (S1 fraction)
indirect immunofluorescence: 5-10 using MDCK cells.
microarray: suitable
western blot: 0.5-1 μg/mL using mouse and rat brain extract (S1 fraction)

UniProt-Hinterlegungsnummer

Q6R891

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PPP1R9B(84687)
mouse ... Ppp1r9b(217124)
rat ... Ppp1r9b(84686)

Immunogen

synthetic peptide corresponding to amino acids 367-390 located at the mid-region of rat neurabin II. The sequence is identical in human and mouse neurabin II and not found in neurabin I.

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

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Linda C Hsieh-Wilson et al.
The Journal of biological chemistry, 278(2), 1186-1194 (2002-11-06)
Spinophilin is a protein phosphatase 1 (PP1)- and actin-binding protein that modulates excitatory synaptic transmission and dendritic spine morphology. We report that spinophilin is phosphorylated in vitro by protein kinase A (PKA). Phosphorylation of spinophilin was stimulated by treatment of
Madepalli K Lakshmana et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(5), 2072-2083 (2012-02-02)
We previously reported that RanBP9 binds low-density lipoprotein receptor-related protein (LRP), amyloid precursor protein (APP), and BACE1 and robustly increased Aβ generation in a variety of cell lines and primary neuronal cultures. To confirm the physiological/ pathological significance of this
H Nakanishi et al.
The Journal of cell biology, 139(4), 951-961 (1997-12-31)
We purified from rat brain a novel actin filament (F-actin)-binding protein of approximately 180 kD (p180), which was specifically expressed in neural tissue. We named p180 neurabin (neural tissue-specific F-actin- binding protein). We moreover cloned the cDNA of neurabin from
Pranay Bharadwaj et al.
PloS one, 8(7), e69672-e69672 (2013-07-23)
Male sexual behavior (MSB) is modulated by gonadal steroids, yet this relationship is highly variable across species and between individuals. A significant percentage (~30%) of B6D2F1 hybrid male mice demonstrate MSB after long-term orchidectomy (herein after referred to as "maters")
C L Pang et al.
Oncogene, 33(31), 4039-4049 (2013-10-22)
High-risk human papillomaviruses are causative agents of cervical cancer. Viral protein E7 is required to establish and maintain the pro-oncogenic phenotype in infected cells, but the molecular mechanisms by which E7 promotes carcinogenesis are only partially understood. Our transcriptome analyses

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