MAK506

Peroxidase Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(e): Peroxidase Enzyme Activity Kit
• UNSPSC-Code: 12352204
• NACRES: NA.84

Eigenschaften

• Qualitätsniveau: 100
• Nachweisverfahren: colorimetric; fluorometric
• Relevante Krankheit(en): cancer; cardiovascular diseases; neurological disorders
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Peroxidase catalyze the following oxidationreduction reactions: ROOR′ + electron donor (2 e-) + 2H+ → ROH + R′OH For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi, and bacteria (cytochrome-c peroxidase like enzymes such as horseradish peroxidase).

Leistungsmerkmale und Vorteile

• Linear detection range: 2 to 50 U/L for the colorimetric assay and 0.1 to 5 U/L for the fluorometric assay.
• Compatible with high-throughput handling systems.

Eignung

The kit is suitable for peroxidase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

Prinzip

The Peroxidase Assay Kit uses H2O2 and an electron donor dye that forms resorufin during the peroxidase reaction. The optical density at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is a direct measure of the enzyme activity.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1