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Merck

MAK199

Sigma-Aldrich

Acetoacetate Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(e):

AcAc Assay Kit

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

endocrinological disorders, diabetes; cancer

Lagertemp.

−20°C

Allgemeine Beschreibung

Acetoacetate (AcAc) is a β-ketoacid formed in the mitochondria by the condensation of two molecules of acetyl-CoA. AcAc is either enzymatically reduced to 3-hydroxybutyrate or decarboxylated to produce acetone. Ketone bodies (AcAc, 3-HB, and acetone) are used as alternate source of energy in the absence of glucose. Excess ketone bodies are observed in type I diabetes, alcoholism, or starvation.

Eignung

Suitable for the detection of acetoacetate in samples such as serum, plasma, blood, urine, and other body fluids.

Prinzip

The Acetoacetate Colorimetric Assay kit provides a simple and sensitive procedure for measuring AcAc in various samples (ranging from 20-100 nmole/well). AcAc concentration is determined by generating a colorimetric (550 nm) product proportional to the amount of AcAc present. The kit does not detect 3-hydroxybutyrate and is specific for acetoacetate.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • Acetoacetate Assay Buffer

  • Acetoacetate Standard

  • Acetoacetate Substrate

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Acute Tox. 4 Oral

Lagerklassenschlüssel

10 - Combustible liquids


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Sensors (Basel, Switzerland), 21(14) (2021-07-25)
In this report, we present an enzyme deposited Au electrode for an electrochemical measurement of acetylacetic acid (AcAc) in urine. The electrode has an immobilized layer of a mixture of D-β-hydroxybutyrate dehydrogenase (HBDH) and nicotinamide adenine dinucleotide (NADH) as sensing
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The Journal of pharmacology and experimental therapeutics, 361(2), 292-302 (2017-02-18)
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