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MAK070

Sigma-Aldrich

Oxaloacetate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Nachweisverfahren

colorimetric
fluorometric

Relevante Krankheit(en)

cancer

Lagertemp.

−20°C

Allgemeine Beschreibung

Oxaloacetate (OAA) is an intermediate of both the TCA cycle and gluconeogenesis. It precedes citrate which is formed by the citrate synthase-mediated condensation of acetyl-CoA and OAA. OAA is also formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl-CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. The Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples.

Anwendung

Oxaloacetate Assay Kit has been used to determine the concentration of oxaloacetate in samples.

Eignung

Suitable for animal tissues, cell culture (adherent or suspension cells), serum and saliva.

Prinzip

In this kit, oxaloacetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the oxaloacetate present. Typical detection ranges for this kit are 2–10 nmole (colorimetric) and 0.2–1 nmole (fluorometric).

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazard
GHS08

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
6J11K06590
5G15K06590
5A16K06590
4G20K06590
3C24K06590

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