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Merck

MAK023

Sigma-Aldrich

Chloride Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric tests

Anwendung(en)

cosmetics
food and beverages

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

pulmonary disorders

Lagertemp.

room temp

Allgemeine Beschreibung

Chloride is an essential anion needed for the proper functioning of many critical aspects of metabolism, including aiding in maintenance of the body′s acid-base balance. In Cystic Fibrosis, mutations to the CF transmembrane conductance regulator (CFTCR) gene result in altered sodium and chloride ion transport channels.

Anwendung

Chloride Assay Kit has been used to measure chloride concentration spectrophotometrically and is used to measure chloride levels in blood plasma of toads Rhinella marina.

Eignung

Suitable for the quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water

Prinzip

The Chloride Assay kit provides a simple and direct procedure for measuring chloride in a variety of samples, including blood and urine. Chloride concentration is determined by a competition reaction between Hg2+ and Fe2+ for 2,4,6-Tris(2-pyridyl)-s-triazine(TPTZ). The preferred Hg-TPTZ complex exhibits no color. In the presence of chloride, Hg2+ forms HgCl2, which precipitates, allowing TPTZ to complex with Fe2+. The Fe-TPTZ complex results in a colorimetric (620 nm) product proportional to the chloride present.

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

H-Sätze

P-Sätze

Gefahreneinstufungen

Aquatic Chronic 3

Lagerklassenschlüssel

10 - Combustible liquids


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