M8159

Monoklonale Anti-MAP-Kinase, aktiviert (Diphosphorylierter ERK-1&2) in Maus hergestellte Antikörper

clone MAPK-YT, ascites fluid

• Synonym(e): Anti-DP-ERK aus mouse
• UNSPSC-Code: 12352203
• eCl@ss: 32160410
• NACRES: NA.44

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: primary antibodies
• Klon: MAPK-YT, monoclonal
• Mol-Gew.: antigen ERK-1 44 kDa; antigen ERK-2 42 kDa
• Enthält: 15 mM sodium azide
• Speziesreaktivität: human, Caenorhabditis elegans, Xenopus, Drosophila, hamster, rat, bovine, mouse, yeast
• Methode(n): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1:10,000 using rat brain extract
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P28482
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MAPK1(5594) , MAPK3(5595) ; mouse ... Mapk1(26413) , Mapk3(26417) ; rat ... Mapk1(116590) , Mapk3(50689)

Beschreibung

Allgemeine Beschreibung

MAP kinase (MAPK, mitogen-activated protein kinase is also termed as ERK, extracellular regulated protein kinase). Molecular cloning has established that MAP kinase (ERKs) consists of at least four isoforms: ERK-1 (p44mapk), ERK-2 (p42mapk), ERK-3, and ERK-5. MAP kinase isoforms appear to be widely expressed in the central nervous system, thymus, spleen, heart, lung and kidney. It is also expressed at high levels in PC12 cells and in fibroblasts. MAPK1 is located on human chromosome 22q11. MAPK3 is mapped to human chromosome 16p11.

Spezifität

Der Antikörper reagiert spezifisch mit der diphosphorylierten Form von MAP-Kinase (ERK-1 und ERK-2). Er erkennt weder die nicht phosphorylierte noch die monophosphorylierte Form von MAP-Kinase oder die diphosphorylierten Formen von JNK und p38-MAP-Kinase. Das vom Antikörper erkannte Epitop enthält die phosphorylierten Threonin- und Tyrosin-Reste mit der Regulierungsstelle von aktiver MAP-Kinase.

Immunogen

Synthetisches Peptid HTGFLpTEpYVAT, das der phosphorylierten Form der ERK-Aktivierungsschleife entspricht.

Anwendung

Antibodies that react specifically with the active form of MAP kinase are useful for the study of the specific activation requirements, differential tissue expression, and intracellular localization of the active form of MAP kinase in normal and neoplastic tissue.
Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) may be used for the localization of the active, dually-phosphorylated, form of MAP kinase using various immunochemical assays such as immunoblotting of cultured cells and tissue extracts, ELISA, immunocytochemistry, immunoprecipitation, and in immunohistochemistry (formalin and formaldehyde-fixed sections). Reactivity has been observed with human, bovine, rat, mouse, Drosophila, Spodoptera frugiperda, and yeast.

Monoclonal Anti-MAP Kinase, activated (Diphosphorylated ERK-1&2) antibody has been used in plasmid and transient transfection and western blotting.

Biochem./physiol. Wirkung

MAP kinase (MAPK, mitogen-activated protein kinase) plays a crucial role in various signal transduction pathways leading signals of growth factor, as well as G protein-coupled receptors to their intracellular targets. MAP kinase was shown to regulate several cellular processes among them proliferation, differentiation, cellular morphology and oncogenesis. Activation of ERK-1 and ERK-2 in mitogen-stimulated cells is directly mediated by MAP kinase kinase (MAPKK or MEK), a dual-specificity protein kinase, which phosphorylates both threonine and tyrosine residues in the regulatory sites of MAP kinase. Following activation, MAP kinase phosphorylates several nuclear targets, including transcription factors as well as membrane and cytoskeletal proteins. Termination of MAP kinase signalling appears to be mediated by MAP kinase phosphatase, MKP-1, a dual specificity Thr/Tyr phosphatase which dephosphorylates and inactivates MAP kinase.

Physikalische Form

The product is provided as ascites fluid containing 15 mM sodium azide as a preservative.

Lagerung und Haltbarkeit

Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable