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Merck

L8262

Sigma-Aldrich

Lektin aus Ulex europaeus (Ginster, Stechginster) aus Ulex europaeus (gorse, furze)

biotin conjugate, lyophilized powder

Synonym(e):

-NotTranslated-

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

Konjugat

biotin conjugate

Qualitätsniveau

Form

lyophilized powder

Wirksamkeit

<50 μg/mL agglutination activity

Zusammensetzung

Protein, ~85% E1%/280

Kennzeichnungsgrad

~3 mol biotin per mol protein

Lagertemp.

2-8°C

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Anwendung

Lectin from Ulex europaeus (gorse, furze) has been used:
  • as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution
  • in double staining for endothelial cells for quantitation of blood vessels
  • in histochemical staining of bronchial epithelial cells

Biochem./physiol. Wirkung

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Verpackung

Package size based on protein content.

Verlinkung

Conjugates are prepared from affinity purified lectin.

Physikalische Form

Contains sodium citrate

Hinweis zur Analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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Daisuke Kondoh et al.
Cells, tissues, organs, 203(1), 20-28 (2016-07-18)
Glycoconjugates in the olfactory system play critical roles in neuronal formation, and α1-2 fucose (α1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of α1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This
M Tango et al.
Archives of histology and cytology, 63(1), 81-89 (2000-04-19)
The aggregation of lymphoid cells in the bronchial mucosa has been named the bronchus-associated lymphoid tissue (BALT) and investigated in comparison with the gut-associated lymphoid tissue (GALT). To elucidate precisely the structure and function of the BALT, the present study
Michael Spencer et al.
Diabetes, 62(5), 1709-1717 (2013-01-19)
Fish oils (FOs) have anti-inflammatory effects and lower serum triglycerides. This study examined adipose and muscle inflammatory markers after treatment of humans with FOs and measured the effects of ω-3 fatty acids on adipocytes and macrophages in vitro. Insulin-resistant, nondiabetic
Sarah Waters et al.
Acta neuropathologica communications, 9(1), 144-144 (2021-08-28)
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease involving progressive degeneration of upper and lower motor neurons. The pattern of lower motor neuron loss along the spinal cord follows the pattern of deposition of phosphorylated TDP-43 aggregates. The blood-spinal
Molly E V Swanson et al.
Scientific reports, 10(1), 11693-11693 (2020-07-18)
Current immunohistochemical methods of studying microglia in the post-mortem human brain do not capture the heterogeneity of microglial function in response to damage and disease. We therefore investigated the expression of eight myeloid cell proteins associated with changes in function

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