L5293

Lipopolysaccharide aus Escherichia coli O111:B4

Ready Made solution, 1 mg/mL

• Synonym(e): LPS
• MDL-Nummer: MFCD00164401
• UNSPSC-Code: 12352201
• NACRES: NA.25

Eigenschaften

• Biologische Quelle: Escherichia coli (O111:B4)
• Qualitätsniveau: 200
• Form: aqueous solution
• Konzentration: 1 mg/mL
• Farbe: colorless to faint yellow
• Löslichkeit: water: soluble
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

Lipopolysaccharides from Escherichia coli O111:B4 has been used:

• to stimulate an inflammatory response in porcine liver tissues in-vivo and piglet ileum tissues ex vivo
• to stimulate Ex vivo natural killer(NK) cells and hepatic B cells
• to induce arthritis in a standard rabbit model

Lipopolysaccharide (LPS) sind charakteristische Bestandteile der Zellwand von gramnegativen Bakterien. LPS und seine Lipid-A-Gruppe stimulieren Zellen des angeborenen Immunsystems durch den Toll-like-Rezeptor 4 (TLR4). TLR4 gehört zur Toll-like-Rezeptor-Proteinfamilie, die häufige Pathogen-assoziierte molekulare Strukturen (PAMPs) erkennt.

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics.

Angaben zur Herstellung

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)