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L0288

Sigma-Aldrich

Lipid Mixture 1, Chemically Defined

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(e):

ACF lipid mixture

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About This Item

UNSPSC-Code:
12352211
NACRES:
NA.75

Biologische Quelle

non-animal source

Qualitätsniveau

500

Sterilität

sterile-filtered

Produktlinie

BioReagent

Form

liquid

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

≤10 EU/mL endotoxin

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

Lipid mixture 1, Chemically defined is recommended for use in cell culture at 1 to 10 mL per liter of medium.

Anwendung

Lipid Mixture 1, Chemically Defined has been used:

  • as a component of bovine serum albumin (BSA) containing Bracket-Oliphant (BO) medium for the resuspension of sperm pellet
  • as a supplement in spheroid media for culturing primary human subcutaneous stromal vascular fraction cells
  • to prepare a cell steatosis model for culturing of mouse and human hepatocytes

Sonstige Hinweise

Contains non-animal derived fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic), 0.22 mg/ml cholesterol from New Zealand sheep′s wool, 2.2 mg/ml Tween-80, 70 μg/ml tocopherol acetate and 100 mg/ml Pluronic F-68 solubilized in cell culture water.

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Lagerklassenschlüssel

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WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Aranya Bagchi et al.
The Journal of experimental biology, 221(Pt 13) (2018-05-12)
Weddell and elephant seals are deep-diving mammals, which rely on lung collapse to limit nitrogen absorption and prevent decompression injury. Repeated collapse and re-expansion exposes the lungs to multiple stressors, including ischemia-reperfusion, alveolar shear stress and inflammation. There is no
Md Mazharul Islam et al.
Reproductive medicine and biology, 20(3), 289-298 (2021-07-16)
The present study was undertaken to clarify whether bovine sperm could take up fatty acids (FAs) and produce ATP to maintain linear motility. Frozen bovine semen was thawed in media containing either lipid mixture (LM) or FAs, and sperm motility
Marc P Hübner et al.
PLoS neglected tropical diseases, 14(7), e0008427-e0008427 (2020-07-07)
A major impediment to eliminate lymphatic filariasis and onchocerciasis is the lack of effective short-course macrofilaricidal drugs or regimens that are proven to be safe for both infections. In this study we tested oxfendazole, an anthelmintic shown to be well
Semir Beyaz et al.
Nature, 531(7592), 53-58 (2016-03-05)
Little is known about how pro-obesity diets regulate tissue stem and progenitor cell function. Here we show that high-fat diet (HFD)-induced obesity augments the numbers and function of Lgr5(+) intestinal stem cells of the mammalian intestine. Mechanistically, a HFD induces
Tamara Zaytouni et al.
Nature communications, 8(1), 242-242 (2017-08-16)
Obesity is an established risk factor for pancreatic ductal adenocarcinoma (PDA). Despite recent identification of metabolic alterations in this lethal malignancy, the metabolic dependencies of obesity-associated PDA remain unknown. Here we show that obesity-driven PDA exhibits accelerated growth and a
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Artikel

Linolenic Acid in Cell Culture

Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Palmitic Acid in Cell Culture

How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Oleic Acid in Cell Culture

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

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