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I5635

Sigma-Aldrich

Monoklonaler ANTI-Human-IgG2-Antikörper

clone HP-6014, ascites fluid

Synonym(e):

Monoclonal Anti-Human IgG2

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

mouse

Qualitätsniveau

200

Konjugat

unconjugated

Antikörperform

ascites fluid

Antikörper-Produkttyp

secondary antibodies

Klon

HP-6014, monoclonal

Enthält

15 mM sodium azide

Methode(n)

indirect ELISA: 1:5,000

Isotyp

IgG1

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Monoclonal Anti-Human IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses.

Spezifität

Der Antikörper ist spezifisch für Human-IgG2 und ist mit anderen IgG-Unterklassen nicht reaktiv. Dieser Klon wurde in der IUIS/WHO-Studie als der am vielseitigsten einsetzbare IgG2-spezifische monoklonale Antikörper hervorgehoben.

Anwendung

Monoclonal Anti-Human IgG2 antibody has been used in in enzyme linked immunosorbent assay (ELISA) and in western blotting.
It may also be used:
  • in imprint immunofixation
  • in immunofluorometric assay
  • in hemagglutination
  • in hemagglutination inhibition
  • in particle counting immunoassay
  • in detection of cytoplasmic IgG

Biochem./physiol. Wirkung

Anti-polysaccharide responses are mainly of the IgG2 sub-class while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccharides stimulate an IgG2 response in peripheral blood leukocytes (PBL′s).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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A conserved Leishmania hypothetical protein evaluated for the serodiagnosis of canine and human visceral and tegumentary leishmaniasis, as well as a serological marker for the posttreatment patient follow-up
Ribeiro P A, et al.
Diagnostic Microbiology and Infectious Disease, 92(3), 196-203 (2018)
Identification of immune biomarkers related to disease progression and treatment efficacy in human visceral leishmaniasis
Portela A S B, et al.
Immunobiology, 223(3), 303-309 (2018)
Negative immunodiffusion test results obtained with sera of paracoccidioidomycosis patients may be related to low-avidity immunoglobulin G2 antibodies directed against carbohydrate epitopes
Neves AR, et al.
Clinical and Diagnostic Laboratory Immunology, 10(5), 802-807 (2003)
D Thiagarajan et al.
ACR open rheumatology, 2(6), 344-356 (2020-05-12)
Immunoglobulin M antibodies against phosphorylcholine (anti-PCs) may be protective in atherosclerosis, cardiovascular disease (CVD), and systemic lupus erythematosus (SLE). We study immunoglobulin G1 (IgG1) and immunoglobulin G2 (IgG2) anti-PCs, with a focus on atherosclerosis and SLE. We determined anti-PCs by
A M Walker et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(8), 3278-3282 (1995-04-11)
Several lines of evidence indicate that immunoglobulin-bound prolactin found in human serum is not a conventional complex between an anti-prolactin antibody and prolactin but a different type of association of prolactin with the Fab portion of IgG heavy chains. The
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