H8651

HEPES Natriumsalz

BioXtra, ≥99.0% (titration)

• Synonym(e): 4-(2-Hydroxyethyl)piperazin-1-ethanesulfonsäure Natriumsalz, N-(2-Hydroxyethyl)-piperazin-N′-(2-ethansulfonsäure) Natriumsalz
• Empirische Formel (Hill-System): C₈H₁₇N₂NaO₄S
• CAS-Nummer: 75277-39-3
• Molekulargewicht: 260.29
• EG-Nummer: 278-169-7
• MDL-Nummer: MFCD00036463
• UNSPSC-Code: 12161700
• PubChem Substanz-ID: 24895527
• NACRES: NA.25

Eigenschaften

• Produktlinie: BioXtra
• Assay: ≥99.0% (titration)
• Form: powder
• Verunreinigungen: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• pH-Wert: 10.0-12.0 (1 M in H₂O)
• Nützlicher pH-Bereich: 6.8-8.2
• pKa (25 °C): 7.5
• Löslichkeit: H₂O: 1 M, clear, colorless
• Anionenspuren: chloride (Cl^-): ≤0.05%; sulfate (SO₄^2-): ≤0.05%
• Kationenspuren: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Pb: ≤0.001%; Zn: ≤0.0005%
• Absorption: ≤0.2 at 260 in H₂O at 1 M; ≤0.2 at 280 in H₂O at 1 M
• Anwendung(en): diagnostic assay manufacturing
• SMILES String: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• InChIKey: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Anwendung

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Sonstige Hinweise

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)