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GE29-0491-04

HiTrap® MabSelect SuRe

Cytiva 29-0491-04, pack of 1 mL

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

alkali-stabilized protein A-derived (E. coli)

Verpackung

pack of 1 mL

Hersteller/Markenname

Cytiva 29-0491-04

Verfügbarkeit

not available in North America

Lagerbedingungen

(20% Ethanol)
(20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)

Parameter

42 psi

Bettgröße

7 mm × 25 mm

Bettvolumen

1 mL

Säule ID

7 mm

Matrix

highly cross-linked agarose

Durchschnittliche Teilgröße

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-13.7

working range

3-12

Kapazität

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

Eignung

suitable for bioprocess medium

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Leistungsmerkmale und Vorteile

  • Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
  • Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiTrap is a registered trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva

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3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protokolle

This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from GE Healthcare.

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