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GE28-9782-45

HiScreen, DEAE Fast Flow

Cytiva 28-9782-45

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About This Item

UNSPSC-Code:
47101511
NACRES:
NA.56

ligand

diethylaminoethyl

Beschreibung

Ion Exchanger Type (value)

Form

prepacked column

Quervernetzung

cross-linked

Verpackung

pkg of 1 ea

Hersteller/Markenname

Cytiva 28-9782-45

Lagerbedingungen

(20% Ethanol)

Parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

Methode(n)

liquid chromatography (LC): suitable

Bettgröße

7.7 mm × 100 mm

Bettvolumen

4.7 mL

Säule ID

7.7 mm

Matrix

6% cross-linked agarose

Aktive Matrixgruppe

N+(C2H5)2H functional group

Partikelgröße

45-165 μm

Durchschnittlicher Durchmesser

90 μm

cleaning

2-14

working range

2-12

Eignung

suitable for bioprocess medium

Trenntechnik

anion exchange

Allgemeine Beschreibung

HiScreen DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Anwendung

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Leistungsmerkmale und Vorteile

  • DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

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Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Alle anzeigen

Protokolle

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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