GE17-0440-03

Con-A Sepharose^™ 4B

Cytiva 17-0440-03, pack of 5 mL

• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• ligand: concanavalin A (con A)
• Verpackung: pack of 5 mL
• Hersteller/Markenname: Cytiva 17-0440-03
• Lagerbedingungen: (0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
• Matrix: 4% agarose
• Partikelgröße: 45-165 μm
• Durchschnittlicher Durchmesser: 90 μm
• cleaning in place: 4-9
• working range: 4-6
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Con A Sepharose^™ 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Anwendung

Con A Sepharose^™ is Concanavalin A coupled to Sepharose^™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose^™ is routinely used for separation and purification of glycoProteins, polysaccharides and glycolipids

Leistungsmerkmale und Vorteile

• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Group-specific adsorbent for molecules containing sugars.
• Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
• Con A coupled to Sepharose^™ 4B via cyanogen bromide activation

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids