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GE17-0413-01

HiTrap® Blue High Performance

Cytiva 17-0413-01, pack of 1 × 5 mL

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

Cibacron Blue F3G-A

Verpackung

pack of 1 × 5 mL

Hersteller/Markenname

Cytiva 17-0413-01

Parameter

42 psi

Bettgröße

16 mm × 25 mm

Bettvolumen

5 mL

Säule ID

16 mm

Größe

Durchschnittliche Teilgröße

34 μm

cleaning

3-13

working range

4-12

Allgemeine Beschreibung

HiTrap® Blue HP is prepacked with Blue Sepharose High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.

Anwendung

Blue Sepharose High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.

Leistungsmerkmale und Vorteile

  • Can be used for depletion of albumin from serum.
  • Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
  • The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

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Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protokolle

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

This page shows how to remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from GE Healthcare.

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose 4B, HiTrap Blue HP and Blue Sepharose 6 Fast Flow

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Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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