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Key Documents

GE17-0020-01

Sephadex® G-15

Cytiva 17-0020-01, pack of 100 g

Synonym(e):

Sephadex gel

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About This Item

UNSPSC-Code:
23151817
NACRES:
NA.56

Leistungsmerkmale

autoclavable In wet form (pH 7.0) at 120°C for 30 min

Verpackung

pack of 100 g

Hersteller/Markenname

Cytiva 17-0020-01

Parameter

flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)

Matrix

cross-linked dextran

Partikelgröße

40-120 μm (dry)
60-180 μm (wet)

working range

2-13

Allgemeine Beschreibung

Sephadex® G-15 is well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules > 1500 molecular weight.

Anwendung

Sephadex® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and Low operating pressures are required.

Leistungsmerkmale und Vorteile

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of biological samples, for example peptides, small Proteins, and oligosaccharides larger than 1 500 molecular weight
  • Efficient removal of contaminants such as salts, dyes, and radioactive labels

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sephadex is a registered trademark of Cytiva

Lagerklassenschlüssel

13 - Non Combustible Solids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Sephadex® LH-20

Sephadex® G-25 Fine

Sigma-Aldrich

G2580

Sephadex® G-25

Sephadex® G-25 Coarse

Sigma-Aldrich

G25300

Sephadex® G-25

Sephadex® G-100 Medium

Sigma-Aldrich

G100120

Sephadex® G-100

Protokolle

Performing a Separation with Sephadex®

This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

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