G115050

DMT-dG(dmf)-Phosphoramidit

configured for ABI, configured for (ÄKTA^® and OligoPilot^®)

• Synonym(e): DMT-dG(dmf)-Amidit, N-[(Dimethylamino)methylen]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxyguanosin, 3′-[2-Cyanoethyl-N,N-bis(1-methylethyl)phosphoramidit], N2-Dimethylformamidin-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosin-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidit]
• Empirische Formel (Hill-System): C₄₃H₅₃N₈O₇P
• CAS-Nummer: 330628-04-1
• Molekulargewicht: 824.90
• MDL-Nummer: MFCD00797420
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 329799825
• NACRES: NA.51

Eigenschaften

• Typ: for DNA synthesis
• Produktlinie: Proligo Reagents
• Assay: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Form: powder
• Methode(n): oligo synthesis: suitable
• Farbe: white to off-white
• λ: conforms (UV/VIS Identity)
• Kompatibilität: configured for (ÄKTA^® and OligoPilot^®); configured for ABI
• Nukleosidprofil: base: deoxyguanosine; base protecting group: DMF; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast/standard
• Lagertemp.: -10 to -25°C
• SMILES String: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1
• InChIKey: YRQAXTCBMPFGAN-UJASEYITSA-N

Beschreibung

Allgemeine Beschreibung

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Changing the dG protecting group to the dimethylformamide (dmf) base protecting group enables the rapid synthesis of high-purity, high-yieldoligonucleotide, thus increasing the efficiency of high-throughputproduction.

Key Features of dG(dmf):

• dG(dmf) is deprotected faster than the conventional dG(ib): thedeprotection time in concentrated ammonia is reduced to 2 hours at55 °C or 1 hour at 65 °C
• The dG(dmf)-monomer is especially suitable for G-rich sequences:incomplete deprotection is greatly reduced in comparison with theconventional dG(ib)-monomer
• dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-,dC(bz)- and dT-phosphoramidites
• dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis(except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should beemployed)DMT-dG(dmf) Phosphoramidite is configured for ABI^™ Synthesizers.

Rechtliche Hinweise

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable