FLAGIPT1
FLAG® Immunoprecipitation Kit
Synonym(e):
Anti-ddddk, Anti-dykddddk
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About This Item
UNSPSC-Code:
12352200
NACRES:
NA.32
Empfohlene Produkte
Verwendung
kit sufficient for 50 reactions
Qualitätsniveau
chemische Klasse(n) des Analyten
proteins (FLAG® Affinity Gels)
Methode(n)
immunoprecipitation (IP): suitable
Versandbedingung
wet ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Anwendung
Learn more product details in our FLAG® application portal.
Leistungsmerkmale und Vorteile
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3X FLAG™ peptide provides efficient and gentle elution through direct competition
Sonstige Hinweise
All prodedures to be performed at 2-8°C.
Rechtliche Hinweise
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
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