F8799

Fas/Fc Chimera from mouse

>97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(e): Apo-1, CD95
• MDL-Nummer: MFCD01867037
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf21 cells
• Qualitätsniveau: 200
• Assay: >97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.03-0.1 μg per mL ED₅₀ (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)
• Mol-Gew.: 55 kDa (glycosylated form)
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: P25446
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Fas(14102)

Beschreibung

Allgemeine Beschreibung

Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.

Anwendung

Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).

Biochem./physiol. Wirkung

Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.

Hinweis zur Analyse

Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable