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E9158

Sigma-Aldrich

p3xFLAG-Myc-CMV™-23 Expression Vector

Shuttle vector for transient expression of secreted dual tagged N-terminal 3xFLAG and C-terminal c-myc fusion proteins

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About This Item

UNSPSC-Code:
12352200

Markierung

3X FLAG tagged
c-Myc tagged (C-terminal)

Qualität

for molecular biology

Form

buffered aqueous solution

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

The p3XFLAG-Myc-CMV™-23 Expression Vector is a 4.8 kb derivative of pCMV5 used to establish transient expression of secreted, dual-tagged N-terminal 3XFLAG™ and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. The incorporation of 3XFLAG in the expression vector results in increased detection sensitivity using ANTI-FLAG M2 antibody.

p3XFLAG-Myc-CMV-23 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.

The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody.The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.

Vector Maps and Sequences

Komponenten

p3XFLAG-Myc-CMV™-23 Expression Vector 20 μg (E6026) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
p3XFLAG-CMV™-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Prinzip

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG® and c-myc fusion constructs.

Rechtliche Hinweise

This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
p3xFLAG-Myc-CMV is a trademark of Sigma-Aldrich Co. LLC

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