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Merck

E7033

Sigma-Aldrich

pFLAG-CMV-2 Expression Vector

shuttle vector for intracellular transient expression of N-terminal Met-FLAG

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About This Item

UNSPSC-Code:
12352200

Markierung

FLAG® tagged

Qualität

for molecular biology
shuttle vector for intracellular transient expression of N-terminal Met-FLAG

Form

buffered aqueous solution

Lage der Peptid-Tags

N-terminal

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG® fusion proteins in mammalian cells.

pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.

The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

Komponenten

  • pFLAG-CMV-2 Expression Vector 20 μg (E7398) is supplied as 0.5 mg/ml in 10 mM Tris-HCl pH 8.0 in 1 mM EDTA.
  • pFLAG-CMV-2-BAP Control Plasmid 20 μg (P5100) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Prinzip

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.

Rechtliche Hinweise

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

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