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Merck

D2569

Sigma-Aldrich

Anti-DPPA5 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(e):

Anti-Developmental pluripotency associated 5, Anti-ESG1, Anti-Embryonal stem cell-specific gene 1

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~14 kDa

Speziesreaktivität

mouse, human, rat

Konzentration

~1 mg/mL

Methode(n)

immunohistochemistry: 1:100-1:250
western blot: 1:500-1:1,000

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Angaben zum Gen

Allgemeine Beschreibung

The DPPA5 (developmental pluripotency associated 5) protein is strongly expressed in primordial germ cells, and the expression is down-regulated during germ cell development.

Immunogen

synthetic peptide corresponding to residues 43-60 human DPPA5. The sequence is 85% identical in mouse and rat.

Anwendung

Anti-DPPA5 antibody produced in rabbit is suitable for immunohistochemistry at a working dilution of 1:100 to 1:250 and for immunoblotting at 1:500 to 1:1000.

Biochem./physiol. Wirkung

DPPA5 is specifically and differentially expressed in human cells that have pluripotency and can be used as a marker of pluripotent stem cells.

Physikalische Form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Soo-Kyoung Kim et al.
Stem cells (Dayton, Ohio), 23(4), 458-462 (2005-03-26)
Pluripotent embryonic germ cells (EGCs) can be derived from the culture of primordial germ cells (PGCs). However, there are no reports of gonocytes, following the stage of PGC development, becoming stem cell lines. To analyze the gene expression differences between
Shichao Liu et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 244(4), 619-627 (2015-01-27)
Mammalian first lineage segregation generates trophectoderm (TE) and pluripotent inner cell mass (ICM), which provides an ideal model for studying the mechanisms of maintenance and loss of pluripotency. In mouse, the transcription factor OCT4 restricts to ICM and plays a

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