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D2191

Sigma-Aldrich

Monoclonal Anti-DC-SIGN1 antibody produced in mouse

clone 120507, purified immunoglobulin, lyophilized powder

Synonym(e):

Anti-CD209, Anti-Dendritic Cell-Specific ICAM-3 Grabbing Non-Integrin

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About This Item

MDL-Nummer:
MFCD03701938
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

200

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

120507, monoclonal

Form

lyophilized powder

Speziesreaktivität

human

Methode(n)

flow cytometry: 2.5 μg using 106 cells
immunocytochemistry: 8-25 μg/mL
immunohistochemistry: 8-25 μg/mL using fixed cells and tissue sections
western blot: 1 μg/mL

Isotyp

IgG2b

UniProt-Hinterlegungsnummer

Q9NNX6

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CD209(30835)

Verwandte Kategorien

Allgemeine Beschreibung

DC-SIGN is a type II protein C-type lectin that binds mannose-rich molecules such as ICAM-3 and ICAM-2 in a calcium dependent manner. DC-SIGN, a 44 kDa molecule, is identical to the HIV-1 binding protein.

Spezifität

Recognizes human DC-SIGN on transfected cells and on monocyte derived dentritic cells. The antibody does not react with either parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

Immunogen

purified from NIH/3T3 cells transfected with recombinant human DC-SIGN.

Anwendung

Monoclonal Anti-DC-SIGN1 antibody produced in mouse is suitable for flow cytometry, immunocytochemistry at a working concentration of 8-25 μg/mL, immunohistochemistry at 8-25 μg/mL using fixed cells and tissue sections and western blotting at 1 μg/mL.

Biochem./physiol. Wirkung

DC-SIGN1 is involved in molecular interactions between dendritic cells and resting T-cells. DC-SIGN interacts with viruses such as X4, R5, X4R5 HIV-1 strains, HIV-2, and SIV. It captures HIV-1 through its interaction with the gp120 envelope glycoprotein and promotes efficient infection of CD4+ T cells that co-express chemokine receptors.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

S Pöhlmann et al.
Journal of virology, 75(10), 4664-4672 (2001-04-20)
Dendritic cells (DCs) efficiently bind and transmit human immunodeficiency virus (HIV) to cocultured T cells and so may play an important role in HIV transmission. DC-SIGN, a novel C-type lectin that is expressed in DCs, has recently been shown to
T B Geijtenbeek et al.
Cell, 100(5), 575-585 (2000-03-18)
Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of
T B Geijtenbeek et al.
Cell, 100(5), 587-597 (2000-03-18)
Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of
T B Geijtenbeek et al.
Nature immunology, 1(4), 353-357 (2001-03-23)
Dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs migrate to the secondary lymphoid organs to initiate immune responses. We now show that DC-SIGN, a DC-specific C-type lectin, supports
B M Curtis et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(17), 8356-8360 (1992-09-01)
The binding of the human immunodeficiency virus (HIV) envelope glycoprotein gp120 to the cell surface receptor CD4 has been considered a primary determinant of viral tropism. A number of cell types, however, can be infected by the virus, or bind
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