D0550

3,5-Dinitrosalicylsäure

used in colorimetric determination of reducing sugars

• Synonym(e): 3,5-Dinitro-2-hydroxy-benzoesäure, DNS
• Lineare Formel: (O₂N)₂C₆H₂-2-(OH)CO₂H
• CAS-Nummer: 609-99-4
• Molekulargewicht: 228.12
• Beilstein: 2220661
• EG-Nummer: 210-204-3
• MDL-Nummer: MFCD00007104
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24893243
• NACRES: NA.47

Eigenschaften

• Assay: ≥98% (HPLC and titration)
• Form: powder
• mp (Schmelzpunkt): 168-172 °C (lit.)
• Löslichkeit: H₂O: 50 mg/mL
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
• InChIKey: LWFUFLREGJMOIZ-UHFFFAOYSA-N

Beschreibung

Anwendung

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem./physiol. Wirkung

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Sicherheitshinweise

• Piktogramme: GHS05,GHS07
• Signalwort: Danger
• H-Sätze: H302,H318
• P-Sätze: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Dam. 1
• Lagerklassenschlüssel: 4.1A - Other explosive hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves