CN402-05

Canine Chondrocytes: CnC (Cryovial)

• UNSPSC-Code: 41106514
• NACRES: NA.81

Eigenschaften

• Biologische Quelle: canine articular cartilage (normal)
• Verpackung: pkg of 500,000 cells
• Hersteller/Markenname: Cell Applications, Inc
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 78
• Morphologie: chondrocyte
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): arthritis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Allgemeine Beschreibung

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Canine Chondrocytes (CnC) are derived from normal canine articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, CnC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes and allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease in geriatric dogs, CnC can be a useful tool for veterinary practice.

Canine Chondrocytes (CnC) were utilized to evaluate anti-inflammatory and anti-oxidant activity of Glyco-Flex III (Yáñez, 2008) and trans-pterostilbene (Remsberg, 2008). They were also used in material studies aimed to develop optimal surfaces/scaffolds for engineered tissues, cellular arrays, biosensors and lab-on-chip devices (Wright, 2012; Shaik 2013a,b).

Ursprung der Zelllinie

Cartilage

Anwendung

production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration

Komponenten

Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO

Angaben zur Herstellung

• 1st passage, >500,000 cells in Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 10 doublings

Subkultur-Routine

Please refer to the CnC Culture Protocol.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable