LS 123

94120801, human colon, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: LS 123, 94120801
• Biologische Quelle: human colon
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 63 (2n=46), hypotriploid
• Morphologie: Epithelial
• Produkte: Carcinoembryonic antigen (CEA)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human colon adenocarcinoma

Beschreibung der Zelllinie

Established in 1974 from the second in a series of 3 primary colonic tumours resected from a 65 year old Caucasian female, which was classified as a Duke′s B mucin producing colon adenocarcinoma. LS 123 is aneuploid, fails to grow progressively in nude mice and forms colonies only in enriched semisolid medium developed for tumour stem cells. The cells will grow in either low serum or serum free medium. In chick embryonic skin assays the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness. LS 123 exhibits several properties associated with neoplasia, but have little invasive potential and may represent an early stage in tumour development.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9,13
D16S539: 13
D5S818: 11
D7S820: 9
THO1: 7,8
TPOX: 9
vWA: 16

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat inactivated).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using trypsin 0.25% or trypsin/EDTA; 5% CO₂, 37°C.

Sonstige Hinweise

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