L6.C11

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92102119, rat muscle, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: rat muscle
• Verpackung: tube of 5 μg 92102119-DNA-5UG; pkg of vial of cells 92102119-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat skeletal muscle myoblast

Beschreibung der Zelllinie

A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.

Sonstige Hinweise

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