GS-109-V-34

90110504, human skin, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: GS-109-V-34, 90110504
• Biologische Quelle: human skin
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian skin fibroblast Gardner′s syndrome

Beschreibung der Zelllinie

Derived from a 6 year old Caucasian female with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Normal female karyotype.

Anwendung

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 11,12
D5S818: 12
D7S820: 8,12
THO1: 6,9.3
TPOX: 10,12
vWA: 14,16

Nährmedium

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer should form in a 25cm² in 3-4 days.

Sonstige Hinweise

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