LL24

90073001, human lung, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: LL24, 90073001
• Biologische Quelle: human lung
• Verpackung: tube of 5 μg 90073001-DNA-5UG; pkg of vial of cells 90073001-1VL
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46; diploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human diploid lung

Beschreibung der Zelllinie

Derived from normal tissue taken at post-mortem from the lung of a 5 year old male. An ampoule of cells frozen after one passage and estimated population doubling (PDL) 2 was submitted to the American Type Culture Collection in June, 1977. Derived populations at passage 6 and approximately 14 PDL are available for distribution. Progeny were found to undergo an additional 25 population doublings in longevity trials.

Anwendung

Tumourigenicity studies

Nährmedium

Ham′s F12 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, a further 20 population doublings expected.

Sonstige Hinweise

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