LA-4

90040512, mouse lung, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: LA-4, 90040512
• Biologische Quelle: mouse lung
• Wachstumsmodus: Adherent
• Karyotyp: Unstable, range 38-256 chromosomes
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse A/He lung adenoma

Beschreibung der Zelllinie

Isolated from a urethan-induced lung adenoma of a 28 week old A/He strain mouse. The cells incorporate high levels of 14C-choline into lecithin. C-type particles have been observed in some cultures.

Anwendung

Tumourigenicity studies

Nährmedium

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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