SCC-4

89062002, human tongue, squamous cell carcinoma. Epithelial morphology.

• UNSPSC-Code: 41106514
• Biologische Quelle: human mouth
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Epidermal keratins; 40 kD Keratin
• Rezeptoren: Not specified
• Relevante Krankheit(en): cancer

Eigenschaften

• Produktbezeichnung: SCC-4, 89062002
• Biologische Quelle: human mouth
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Epidermal keratins; 40 kD Keratin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human tongue squamous carcinoma

Beschreibung der Zelllinie

Derived from a human squamous cell carcinoma (SCC) of the tongue from a 55-year-old male. SCC-4 cells have been reported to form colonies in semi-solid medium, and are not induced to differentiate by anchorage deprivation. Growth is enhanced by use of a feeder layer of 3T3 swiss cells.

Nährmedium

DMEM:HAMS F12 (1:1) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 0.4 μg/ml hydrocortisone

Subkultur-Routine

Split confluent cultures 1:3 to 1:4, i.e., seeding at 3-5 x 10,000 cells/cm² using trypsin/EDTA; 5% CO₂; 37°C

Sonstige Hinweise

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