SW480 Cell Line human

87092801

• Synonym(e): SW-480 Cells, SW480E Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human colon
• Beschreibung: Human colon adenocarcinoma
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human colon adenocarcinoma

Beschreibung der Zelllinie

Derived from a grade 3-4 colon adenocarcinoma. The initial cultures contained a mixture of epithelial and bipolar cells, but subsequently epithelial cells predominated. The cells produce CEA.

Anwendung

Human SW480 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used as an in vitro model to study key cellular processes required for metastasis. The cell line is used to study the effects of the transforming growth factor-β (TGF-β) pathway and heat shock protein 90 (Hsp90) on the growth of cells.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
THO1: 8
TPOX: 11
vWA: 16

Nährmedium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin; 37°C. Medium change every 4-5 days. CO₂ is not required.

Sonstige Hinweise

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