Clone M-3

87081806, mouse skin, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: Clone M-3, 87081806
• Biologische Quelle: mouse skin
• Wachstumsmodus: Adherent
• Karyotyp: Hypertetraploid
• Morphologie: Epithelial
• Produkte: Melanin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse (CxDBA)F1 melanoma

Beschreibung der Zelllinie

Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.

Anwendung

Cloning studies

Nährmedium

Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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