KNRK

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87061001, rat kidney, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: rat kidney
• Verpackung: tube of 5 μg 87061001-DNA-5UG; pkg of vial of cells 87061001-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial-like
• Produkte: Growth factors
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Normal rat kidney, Kirsten MSV transformed

Beschreibung der Zelllinie

Derived from normal rat kidney transformed by Kirsten sarcoma virus. Cells do not produce virus but carry the virus genome. In serum free medium the cell line produces growth factors capable of stimulating quiescent cells to grow in monolayers or in soft agar.

Anwendung

Transformation, growth factors and virus studies: Kirsten MSV

Nährmedium

DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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