HEL 299

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87042207, human lung (embryonic), Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human lung (embryonic)
• Verpackung: tube of 5 μg 87042207-DNA-5UG; pkg of vial of cells 87042207-1VL
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, diploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human embryo lung

Beschreibung der Zelllinie

Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. Cells have a limited lifespan of 20 serial subcultures from tissue of origin. Ethnicity: Black.

Anwendung

HEL 299 has been used to study the antiviral properties of AIC246, a novel anti-human cytomegalovirus compound. It has also been used to study the role of SUMO ligase protein inhibitor of activated STAT1 (PIAS1) in the intrinsic antiviral immune response to herpes simplex virus.

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 11,12
D16S539: 10,11
D5S818: 11,13
D7S820: 8,11
THO1: 7
TPOX: 8,12
vWA: 16

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Limited lifespan.

Sonstige Hinweise

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