CV-1

87032605, African green monkey kidney, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: CV-1, from African green monkey kidney, 87032605, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Biologische Quelle: African green monkey kidney
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 60, pseudodiploid
• Morphologie: Fibroblast
• Rezeptoren: Not specified

Beschreibung

Ursprung der Zelllinie

Monkey African Green kidney fibroblast

Beschreibung der Zelllinie

Used in Rous sarcoma virus (RSV) transformation studies. Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. Derived from a male adult African green monkey kidney. Cells grow rapidly and form monolayers of fibroblast-like cells that support the growth of SV40. Chromosome number shifts have been observed at high passage levels.

Nährmedium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37 °C.

Sonstige Hinweise

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