RPMI 8226

87012702, human blood, Not specified

• Synonym(e): RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: RPMI 8226, 87012702
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Immunoglobulin G (Ig) lambda light chain
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human myeloma

Beschreibung der Zelllinie

Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.

Anwendung

RPMI 8226 has been used:

• to grow human myeloma cell lines
• to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
• to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.

Sonstige Hinweise

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