P3HR-1

86010701, human lymph, Lymphoblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: P3HR-1, 86010701
• Biologische Quelle: human lymph
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 46, hyperdiploid
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Burkitt′s Lymphoma

Beschreibung der Zelllinie

Clonally-derived sub-line of JIYOYE (Sigma Catalogue number. 88071302) containing high levels of EBV. Has been successfully hybridised with the human lymphoid cell line K562 to form the hybrid PUTKO-1. ACDP class II pathogen and should be handled under laboratory containment level 2. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19

Nährmedium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.