DAUDI Cell Line human

85011437, human blood, Lymphoblast

• Synonym(e): DAUDI Cell Culture, Human DAUDI Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: DAUDI Cell Line human, 85011437, African-American Burkitt′s lymphoma, from human blood
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 46, diploid
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Burkitt′s lymphoma

Beschreibung der Zelllinie

Derived from a 16 year old male. Positive for EBNA, carries EBV markers, complement receptors, surface bound immunoglobulin and surface markers for the Fc fragment of IgG. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Anwendung

Tumourigenicity and Virus studies: EBV used to study leukemogenesis mechanisms.

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 10,12
D5S818: 8,13
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 15,17

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Will increase 12 fold within 10-14 days provided fresh medium is added at 48-72 hour intervals. Cell growth is slow on recovery from frozen. Resuscitate using 20% FBS and keep culture flask standing up until exponential growth is established.

Sonstige Hinweise

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