MDCK Cell Line canine

84121903

• Synonym(e): MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• UNSPSC-Code: 41106514
• eCl@ss: 32190102

Eigenschaften

• Biologische Quelle: canine kidney
• Form: cell suspension
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 78
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Canine Cocker Spaniel kidney

Beschreibung der Zelllinie

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.

Anwendung

Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Sonstige Hinweise

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