SPC212

11120717

• Synonym(e): SPC 212, SPC-212
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human lung
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epitheloid/mesenchymal
• Rezeptoren: Mesothelial-membrane antigen ME1
• Relevante Krankheit(en): cancer

Beschreibung

Ursprung der Zelllinie

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Beschreibung der Zelllinie

SPC212 was derived from a biphasic tumour of the pleural cavity of a 47-year old female patient, prior to treatment, with a known history of exposure to asbestos.

DNA-Profil

STR-PCR Data:

Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 11,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16

Nährmedium

DMEM:F12 (1:1) (D8062) + L-Glutamine (G7513) + 15% FBS / FCS (F2442).

Subkultur-Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO₂; 37 °C. Suggested seeding density 1-4 x 10,000 cells/cm².

Sonstige Hinweise

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