SPC111

11120716, human lung (effusion), Epitheloid/mesenchymal

• Synonym(e): SPC-111, SPC111
• UNSPSC-Code: 41106514

Eigenschaften

• product name: SPC111, 11120716
• Biologische Quelle: human lung (effusion)
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epitheloid/mesenchymal
• Rezeptoren: Mesothelial-membrane antigen ME1
• Relevante Krankheit(en): cancer

Beschreibung

Ursprung der Zelllinie

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Beschreibung der Zelllinie

SPC111 was derived from the pleural effusion of a 55-year old male patient, prior to treatment, with a known history of exposure to asbestos.

DNA-Profil

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12
D16S539: 11,13
D5S818: 10,13
D7S820: 8,10
THO1: 9.3
TPOX: 9
vWA: 18

Nährmedium

DMEM:F12 (1:1) (D8062) + L-Glutamine (G7513) + 15% FBS / FCS (F2442).

Subkultur-Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO₂; 37 °C. Suggested seeding density 1-4 x 10,000 cells/cm².

Sonstige Hinweise

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