MKL-1

09111801, human skin. Loose floating aggregates with irregular outline and no central necrosis.

• Synonym(e): MKL1 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: MKL-1, from human skin, 09111801
• Biologische Quelle: human skin
• Wachstumsmodus: Suspension
• Karyotyp: Hyperdiploid
• Morphologie: Loosely packed floating aggregates with irregular outline and no central necrosis
• Produkte: MKL-1 is positive for CM2B4/MCV T AE1/AE2, CK20, EMA, CAM5.2, NSE and TTF-1. MKL-1 is negative for CK7, chromagen, synaptophysin, neurofilament, LCA, vimentin and CD99.
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice

Beschreibung

Ursprung der Zelllinie

Human neuroendocrine skin carcinoma

Beschreibung der Zelllinie

MKL-1 was established from a nodal metastasis in a 26-year-old Caucasian male patient. MKL-1 cells contain a Merkel cell polyomavirus integrated into the genome. MKL-1 cells cause tumour formation at 4-6 weeks at subcutaneous sites in athymic male nude mice (BALB/c nu/nu).

DNA-Profil

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 10,12
D5S818: 11,12
D7S820: 8,11
THO1: 9,9.3
TPOX: 8
vWA: 16,18

Nährmedium

RPMI 1640 + 2 mM Glutamine + 10% FBS

Subkultur-Routine

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37 °C Saturation density is approximately 10⁶ cells/ml. Population doubling time is around 120 hours.

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