FTC-236

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6030202, human thyroid (lymph node metastasis), Endothelial

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human thyroid (lymph node metastasis)
• Verpackung: tube of 5 μg 06030202-DNA-5UG; pkg of vial of cells 06030202-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Endothelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Thyroid Cancer Neck Lymph Node Metastasis

Beschreibung der Zelllinie

Derived from a neck lymph node metastasis of a follicular thyroid cancer (FTC).This cell line is tumourigenic in Balb/c mice. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

Nährmedium

DMEM and Ham′s F12 (1:1 mixture) + 10ug/ml Insulin + 0.01U/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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